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Ansari Yaasir Ahmed Razzaq*, Gulam Javed Khan, Ansari Abdul Aleem, Ansari Abubakar, Jamil Ahmad Mohammad Abbas | Abstract

Asian Journal of Pharmaceutical Technology and Innovation (ajpti)

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Ansari Yaasir Ahmed Razzaq*, Gulam Javed Khan, Ansari Abdul Aleem, Ansari Abubakar, Jamil Ahmad Mohammad Abbas

Abstract

Ansari Yaasir Ahmed Razzaq*, Gulam Javed Khan, Ansari Abdul Aleem, Ansari Abubakar, Jamil Ahmad Mohammad Abbas

Ondansetron ODT, most widely used anti-emetic drug, in this regard, the Research and Validation of reliable analytical method for determination. The main aim of present Research work to Validate a method for estimation of Ondansetron ODT by using High Performance Liquid Chromatography. For Chromatographic determination, mixture of Potassium phosphate:acetonitrile(52:48) was used as mobile phase, the column having length of 4.6 mm × 25 cm and containing packing L7 which is maintained at 45 ± 1 Ë??C temperature. The injection volume was 10 μl and the flow rate was 1.5 mL/min. The assay method was validated for analytical parameters such as linearity, accuracy, precision, system suitability, robustness and range. From the results it is concluded that, HPLC is less time consuming and cost reducing and the results obtained are more accurate, precise and reproducible as compared to UV.

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