Antimicrobial Activity of Rose Geranium (Pelargonium roseum) Essential Oil on Bacteria of Veterinary Clinical Origin
Abstract
Bhoj R Singh*1, Ravi Kant Agrawal2, Sakshi Dubey1, Monika Bhardwaj1, Prasanna Vadhana1
In the study on antimicrobial activity of rose geranium oil (GEO), 167 bacterial strains belonging to 22 genera and 49 species were tested. In total only seven strains were sensitive to GEO. Out of 15 reference strains included in the study only one (Streptococcus equi ssp. equi MTCC-3522) and six of the 120 isolates from clinically sick animals were found sensitive. None of the 26 isolates from environment (soil, water and air) and six from healthy animals was sensitive to GEO. All resistant strains (160) had minimum inhibitory concentration (MIC) more than 2μL of GEO / mL of medium. Sensitive strains’ GEO MIC ranged between 0.2 μL/ mL to 2μL/ mL. The minimum MIC was for Pasteurella langaensis (0.2 μL/ mL) strain followed by strain of Streptococcus equi ssp. equi MTCC-3522 (0.4μL/ mL), Strept. intestnalis and Strept. pyogenes (0. 4μL/ mL), Strept. equi ssp. equi (0.8μL/ mL), Bacillus polymyxa (1.5 μL/ mL) and Pseudomonas aeruginosa (2.0μL/ mL). The study emphasizes need for evolution of some short of central universally accepted guidelines to perform and report antimicrobial activity of herbal antimicrobials so that the comparable data can be analyzed for future meta-analytical and clinical purposes.
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