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Development and Validation of UV Spectrophotometric Method and RP ├ó┬?┬? HPLC Method for Simultaneous Estimation of Teneligliptin and Pioglitazone In Synthetic Mixture

Abstract

Jinal Alkesh Gheewala*, Dilip Girish Maheshwari

The present Article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and RP-HPLC method for the simultaneous estimation of Teneligliptin and Pioglitazone in Synthetic Mixture. In The first order derivative method absorption at 228.5 nm (zero crossing point for Pioglitazone) was used for Teneligliptin and 269.2 nm (zero crossing point for Teneligliptin) was used for Pioglitazone. The linearity was taken in the concentration range of 2-10 μg/ml for Teneligliptin and 3-15 μg/ml for Pioglitazone with correlation coefficient (R2) 0.995 and 0.997, respectively. For The RP-HPLC method linearity was taken in the concentration range of 1- 5 μg/ml for Teneligliptin and 1.5-7.5 μg/ml for Pioglitazone with correlation coefficient (R2) 0.998 and 0.996, respectively. Proposed technique has been validated as per ICH guideline and successfully applied to the simultaneous estimation of Teneligliptin and Pioglitazone in their Synthetic Mixture. The results of analysis have been validated statistically and by recovery studies.

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