Development and Validation RP-HPLC Method For Simultaneous Estimation of Cefuroxime Axetil and Linezolid In Combined Dosage Form
Abstract
Kinjal A. Patel*, Jignesh S. Shah1, Dilip G. Maheshwari2
A simple, specific and accurate Reverse Phase High Performance Liquid Chromatography Method was developed for the simultaneous determination of Cefuroxime Axetil and Linezolid in combined dosage form. The using Kromasil C-8 (250×4.6 mm, 5 μm) column in Isocratic mode, with Mobile Phase Acetonitrile : Phosphate buffer, pH=4.0 (60:40 % v/v) (pH 4.0 adjusted with Orthophosphoric acid). The Flow Rate was 0.9 ml/min and effluents were monitored at 268 nm. The Retention Time of were found to be Linezolid and Cefuroxime Axetil 6.860 min and 8.840 min respectively. The Linearity for Cefuroxime Axetil and Linezolid were found to be 2.5-12.5 μg/ml and 3-15 μg/ml respectively. The Recoveries of Cefuroxime Axetil and Linezolid were found to be 101.66 – 101.90% and 98.14 - 101.15 % respectively. The proposed method was validated and successfully applied for the estimation of Cefuroxime Axetil and Linezolid in combined dosage form.
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