Effect of UV-C on Morus Macroura-Mediated Biosynthesis and Clinical Activities of Metallic (Ag and ZnO) and Bimetallic (Ag-ZnO) Nanoparticles
Nano-revolution based on the green synthesis of nanomaterials is uprising all zones of human life, modeling nanotechnology a propitious platform for various biomedical applications. In this study, biocompatible monometallic (AgNPs and ZnONPs) and bimetallic Ag-ZnONPs were synthesized by using the leave extract of Morus Macroura under the irradiation of UV-C light and were further characterized and evaluated for their anti-diabetic and anti-glycation activities. Ultraviolet-visible spectroscopy of synthesized NPs presented a distinctive surface plasmon bands for all types of synthesized NPs. SEM analysis revealed a variety of shapes and sizes of control and UV-mediated NPs. FTIR showed the presence of polyphenols, carboxylic acid and amines that were primarily accountable for reduction and stabilization of synthesized NPs. In addition, EDX study gave strong justifications of the productive formation of NPs. UV-C mediated NPs showed well defined characteristic shapes with more dispersed particle distribution and reduced size as compared to their respective controls. In case of clinical activities, the highest anti-diabetic activity (67.77 ± 3.29 against α-amylase and 36.43 ± 2.77 against α-gulcosidase) and anti-glycation activity (37.68 ± 3.34 against Pentosidine-Like AGEs and 67.87 ± 2.99 against Vesperlysine-Like AGEs) was shown by UV-mediated AgNPs. Whereas, the highest biocompatibility (IC50=24.13 ± 1.68 µg/ml against Brine Shrimp and 2.48 ± 0.32 % hemolysis of human red blood cells) was shown by UV-mediated ZnONPs. The present work highlighted the positive effects of UV-C light on the physico-chemical properties as well clinical activities of NPs.
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