Estimation of Gallic Acid in the Seed Extracts of Syzygium cumini
Amudha S*, P. K. Manna1, Jeganathan N.S, Anbazhagan S
The aim of this work was to estimate the amount of gallic acid present in raw seed powder, and in the aqueous and alcoholic extracts of Syzygium cumini seed by using reverse phase High pressure liquid chromatography. The method was carried out on a Phenomenex kromosil C18 column (250 × 4.6 mm, 5μ) using mobile phase of 2ml orthophosphoric acid in 1000ml water and methanol with a gradient elution at the flow rate of 1 ml/min, with the detection wavelength at 272 nm. The linear regression analysis data for the calibration showed a good linear relationship with correlation co-efficient of 0.999. The developed method has been validated as per ICH guidelines and the method has the advantage of being both rapid and easy and hence it can be applied for routine quality control analysis of herbal preparations containing Syzygium cumini constituents.
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