Self-assembling peptide nanofibers in bone tissue engineering
Bone is a real nanocompsite of nanofibers and nano ceramics and approximately 600,000 suffers from craniofacial deficits in US. RADA as a core of self-assembling peptides exhibits an acidic pH while the pH of KSL is higher than RADA. The acidic pH of RADA usually is an obstacle in tissue engineering but by regards to the acidophilic nature of bone, it was investigated for the first time. In the present investigation, for the first time the BMHP motif was bound to the RADA and KSL as a core of self-assembling peptide nanofiber and was evaluated its cell viability, ROS, NO and LDH release on MG-63 cell line as a cell line of bone osteosarcoma and then its effects was evaluated as a gene expression of apoptotic and integrin. Then, they were implanted in a critical size bone defect in rats for 2 month and densitometry of bone defects were analyzed and compared.
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